MultiLED Light Source

• Four channel
• USB control
• Intelligent LED heads

Product Description

Introducing our latest and finest LED light source

New four channel version of our OptoLED range!

The MultiLED includes USB control and a graphical user interface as standard.  LED heads now include memory chip to set and show LED information and operating parameters on a built-in alphanumeric display.

“Boost” mode available for temporary LED overdriving while maintaining protection and programmable timers with millisecond resolution and microsecond accuracy now included.

The option of combining these devices with optical feedback control continues for the MultiLED.  This offers key advantages for live cell recordings where short and long term intensity variations may be a problem without this facility.  The ultra-high stability and near-instantaneous (just a few microseconds) vibration-free switching and intensity modulation are valuable in a wide range of applications such as combined imaging and electrophysiology, voltage sensitive dye studies and high-speed tracking.  The facility to “gate” the light source directly from the camera and thus only expose the specimen while the camera is integrating helps reduce photobleaching/photodamage and also prevents bleedthrough during frame readout or rolling shutter artefacts.  Ultraviolet and white LEDs are also available for uncaging applications and brightfield illumination.  The rapid modulation and ability to accurately define independent illumination areas for different wavelengths make both the OptoLED and the new MultiLED the ideal sources for Optogenetics.  A built-in programmable timer function is also now incorporated, which allows these and other applications to be controlled entirely within the light source itself.  Also the control range is now automatically matched to the maximum continuous current rating for each LED, while at the same time allowing an optional “boost” facility for transient overdriving.

Available Wavelengths

The range of wavelengths available from ultra-bright LEDs covers the absorbance peaks of most fluorescence indicators and Optogenetic tools. The table below shows the standard range, however we can also source more specialist devices on request.

Depending on the combination of wavelengths required we can combine two or more devices using dichroic mirrors. Please contact Cairn for recommendations on the best LEDs and filters for your experiments.

OptoLED Available Wavelengths

MultiLED Data Sheet Manuals Get a Quote Comparisons Back to LED Light Sources

Additional information

Applications

• Multi-wavelength fluorescence microscopy
• Visible/IR transmitted light microscopy
• Optogenetics
• Flash photolysis
• Dedicated dual LED heads available for fura-2 or other dual excitation dyes

Key Benefits

• New four channel version of our OptoLED range
• USB control and Graphical User Interface included as standard
• Built-in alphanumeric display to show LED information and operating parameters at a glance
•Uses our existing modular LED head design for full compatibility with our other LED products
• Wide variety of LED wavelengths available, from UV to IR. If it exists, we can provide it!
• LED heads continue to optionally include optical feedback for near perfect stability over both long and short timescales
• LED heads can now include memory to set and show LED information and operating parameters
• Fast (<10 microsecond) switching via external digital inputs
• Similarly fast analog intensity control via front panel, external input or USB (but subject to normal USB latency times in that case)
• Tenfold lower range provided for more precise control of low intensities
• “Boost” mode available for temporary LED overdriving while maintaining protection
• Programmable timers with millisecond resolution and microsecond accuracy now included
• Four additional timer channels provided for controlling other equipment
• Modular LED coupling system now extended to include compact lower-cost version for easier direct connection to a microscope, especially suitable for four-channel use

Full Specification

• Powerful USB interface and GUI for computer control, but not essential for normal use
Maximum current per channel up to 5A, automatically set to the safe maximum for each LED
• External control voltage range 0-5V, matching the set current range for each LED
• LED currents can be set under USB control to 0.1% precision, or optionally 0.025%
USB control also allows selection of a tenfold lower current range for both USB and analog input levels
• Digital control inputs compatible with both 5V and 3.3V TTL levels
• Optical switching times <10usec, typically around 5usec, for both analog and digital control
• Independent timer channel available for each LED, plus four auxiliary channels for possible control of other equipment
• Timer range 0-10 seconds at 1msec precision with <10usec reproducibility
• Timers can either recycle continuously or run in response to a digital trigger
• Boost mode for up to 2x transient overdriving while maintaining LED protection
• Continuing modular LED head design allows us to fit any available LED wavelength on request.
• Direct LED head coupling to most microscopes avoids the need for loss-inducing light guides
• Coupling range now includes a new compact and easy to use system, especially suitable for multiple LED applications

Testimonials

Our need was to stimulate an isolated photoreceptor cell with sufficient light intensity to isomerize nearly all the photopigment molecules, and do so with the shortest flash duration possible: this would synchronize all such events, summate their electrical effects to maximize detectabililty, and avoid overlap with the photocurrent that is elicited only a few milliseconds later. Even the fastest electromechanical shutters proved inadequate for this task, while discharge flash lamps produced unacceptable electrical interference. We were pleased that the OptoFlash (OptoLED) could pump enough photons within a sub-millisecond flash to even saturate rhodopsin, maintaining an excellent intensity control and defined time course.
Enrico Nasi – Cellular dynamics, Woods Hole, MBL

Additional information

Related articles

Why synchronisation with the camera matters

Why can LEDs benefit from optical feedback

Recent publications

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