Improved objective lens, light source, camera and optical filter technology have made high-speed and extended timelapse live cell fluorescence imaging both affordable and accessible to more research groups. Measurement of membrane potential using voltage sensitive dyes still requires specialist knowledge and equipment (which we are happy to provide), but calcium imaging and fast morphological studies at multiple wavelengths can be achieved with relatively inexpensive hardware and software, and with straightforward experimental protocols. At the other extreme of the temporal range, hardware autofocus, LED feedback illumination, and full environmental chambers makes it possible to conduct experiments over several days.
We can help configure systems on new or existing inverted and upright microscopes, advising on all components and providing as many or as few as you need. Systems can be built up over time and functionality improved in line with your scientific requirements and budget. Please talk to us, we are happy to advise, and if we can’t help then we will direct you to someone who can.