LED light source | Phase Contrast Illuminator
Ultra long-distance phase contrast for inverted microscopes
The Cairn Aura phase contrast illuminator allows true Zernike phase contrast with a working distance up to 180 mm (clear working space!) for your most demanding experiments. Based on a concept developed by Webb et al. at the University of Nottingham, the Aura uses concentric rings of super-bright LEDs in place of the conventional condenser and phase rings. The Aura provides the highest-quality phase contrast images with very simple alignment and works with a selection of inverted research microscopes for electrophysiology, live cell imaging, and multimodal time
lapse experiments. The Aura allows turn-key PhL, Ph1, and Ph2 and some darkfield imaging in white light or the near infrared (other colours on request).
* Condenser-free Zernike phase contrast imaging for scanning probe microscopy – Webb KF, de Fillipi G, Johnston NS. Zernike phase contrast microscopy for scanning probe instruments. Microscopy and Analysis (EU) 27(5):27-32, (2013)
Condenser-free contrast methods for transmitted-light microscopy – Webb, K. F., Condenser-free contrast methods for transmitted-light microscopy. Journal of Microscopy, 257: 8–22. doi:10.1111/jmi.12181 (2015)
Fig. 1. (A) Optical schema for conventional Zernike phase contrast microscopy, consisting of collimated light source, phase annulus, condenser assembly and phase contrast objective lens. Inset view of objective BFP via Bertrand lens showing overlapped phase annulus and phase ring. (B) Optical schema for condenser-free Zernike phase contrast consisting of LED ring and phase contrast objective. Inset view of objective BFP showing overlapped image of LED