Affordable Motorised Total Internal Reflection Fluorescence and Widefield Illumination
The OptoTIRF V2 is a compact and powerful, yet inexpensive, stepper-motor-controlled TIRF illuminator designed to fit onto any research-grade inverted microscope. It allows a single or multimode laser spot to be focussed anywhere in the back-aperture of an objective lens with joystick or software control, and simple storage and recall of preset positions via digital or COM interface. This makes it suitable for acquisition protocols involving TIRF and / or oblique illumination at a range of penetration depths and wavelengths. Although it lacks the fast scanning functionality of the Gataca iLAS (which we are also proud to distribute), the 360 degree stepper-motor-control does allow the user to illuminate from multiple points during an experiment, making it straightforward to tweak the illumination to the sample and minimise fringes or shading gradients. Field uniformity is further enhanced by a dither function that helps to avoid the artefacts associated with point TIRF. Flexibility is enhanced by a motorised bypass port, for widefield illumination using LEDs or a liquid light guide, or, for the addition of a second TIRF module for truly simultaneous dual-colour imaging.