Which camera to go with my microscope objective?
Objective Magnification | Objective NA | XY Resolution limit for 500nm light (nm) | Theoretical Pixel size for Nyquist 1/2 Raleigh (um) |
R1 | R6 | sCMOS 4.2 | sCMOS 5.5 | 512 EMCCD | |||||
Pixel/µm | Field of view/µm | Pixel/µm | Field of view/µm | Pixel/µm | Field of view/µm | Pixel/µm | Field of view/µm | Pixel/µm | Field of view/µm | ||||
1 | 0.1 | 3050 | 1.53 | 6.45 | 8,800×6,600 | 4.54 | 12,200×10,000 | 6.5 | 13,300×13,300 | 6.5 | 16,600×14,000 | 16 | 12,200×10,000 |
1 | 0.25 | 1220 | 0.61 | 6.45 | 8,800×6,600 | 4.54 | 12,200×10,000 | 6.5 | 13,300×13,300 | 6.5 | 16,600×14,000 | 1 | 2,687×2,203 |
2 | 0.2 | 1525 | 1.53 | 6.45 | 4,400×3,300 | 4.54 | 6,100×5,000 | 6.5 | 6,500×6,500 | 6.5 | 8,300×7,000 | 2 | 2,687×2,203 |
2 | 0.5 | 610 | 0.61 | 6.45 | 4,400×3,300 | 4.54 | 6,100×5,000 | 6.5 | 6,500×6,500 | 6.5 | 8,300×7,000 | 2 | 2,687×2,203 |
4 | 0.2 | 1525 | 3.05 | 6.45 | 2,200×1,650 | 4.54 | 3,050×2,500 | 6.5 | 3,325×3,325 | 6.5 | 4,150×3,500 | 5 | 2,687×2,203 |
5 | 0.5 | 610 | 1.53 | 6.45 | 1,760×1,320 | 4.54 | 2,440×2,000 | 6.5 | 2,660×2,660 | 6.5 | 3,320×2,800 | 6 | 2,687×2,203 |
10 | 0.45 | 678 | 3.39 | 6.45 | 880×660 | 4.54 | 1,220×1,000 | 6.5 | 1,330×1,330 | 6.5 | 1,660×1,400 | 11 | 2,687×2,203 |
16 | 0.8 | 381 | 3.05 | 6.45 | 550×413 | 4.54 | 763×625 | 6.5 | 831×831 | 6.5 | 1,038×875 | 18 | 2,687×2,203 |
20 | 0.75 | 407 | 4.07 | 6.45 | 440×330 | 4.54 | 610×500 | 6.5 | 665×665 | 6.5 | 830×700 | 23 | 2,687×2,203 |
20 | 1 | 305 | 3.05 | 6.45 | 440×330 | 4.54 | 610×500 | 6.5 | 665×665 | 6.5 | 830×700 | 23 | 2,687×2,203 |
25 | 1.1 | 277 | 3.47 | 6.45 | 352×264 | 4.54 | 488×400 | 6.5 | 532×532 | 6.5 | 664×560 | 28 | 2,687×2,203 |
40 | 0.95 | 321 | 6.42 | 6.45 | 220×165 | 4.54 | 305×250 | 6.5 | 333×333 | 6.5 | 415×350 | 45 | 2,687×2,203 |
40 | 1.1 | 277 | 5.55 | 6.45 | 220×165 | 4.54 | 305×250 | 6.5 | 333×333 | 6.5 | 415×350 | 45 | 2,202×1,805 |
40 | 1.3 | 235 | 4.69 | 6.45 | 220×165 | 4.54 | 305×250 | 6.5 | 333×333 | 6.5 | 415×350 | 45 | 2,687×2,203 |
60 | 1.2 | 254 | 7.63 | 6.45 | 147×110 | 4.54 | 203×167 | 6.5 | 222×222 | 6.5 | 277×233 | 68 | 2,687×2,203 |
60 | 1.3 | 235 | 7.04 | 6.45 | 147×110 | 4.54 | 203×167 | 6.5 | 222×222 | 6.5 | 277×233 | 68 | 2,687×2,203 |
60 | 1.4 | 218 | 6.54 | 6.45 | 147×110 | 4.54 | 203×167 | 6.5 | 222×222 | 6.5 | 277×233 | 68 | 2,687×2,203 |
60 | 1.49 | 205 | 6.14 | 6.45 | 147×110 | 4.54 | 203×167 | 6.5 | 222×222 | 6.5 | 277×233 | 68 | 2,687×2,203 |
100 | 1.45 | 210 | 10.52 | 6.45 | 88×66 | 4.54 | 122×100 | 6.5 | 133×133 | 6.5 | 166×140 | 114 | 2,687×2,203 |
100 | 1.49 | 205 | 10.23 | 6.45 | 88×66 | 4.54 | 122×100 | 6.5 | 133×133 | 6.5 | 166×140 | 114 | 2,687×2,203 |
150 | 1.45 | 210 | 15.78 | 6.45 | 59×44 | 4.54 | 81×67 | 6.5 | 89×89 | 6.5 | 111×93 | 170 |
2,687×2,203
|