Optosplit III

• Triple emission image splitter
• 1, 2 or 3 images on a single camera
• Large sensor sCMOS format

Product Description

The Optosplit III (TripleSplit) extends the OptoSplit image splitter concept, adding an optional second beamsplitter to split the field into either two or three separate, spatially equivalent, channels, which can be displayed side by side on a single camera chip.

Splitting is usually performed on the basis of wavelength or polarisation, allowing applications where there is a requirement for simultaneous, or high speed, acquisition of multiple emission bands or polarisations states. The simultaneous acquisition of up to three images offers a major benefit over manual or electronic filter changers, as there is no longer a need to pause acquisition while the filter position is changed. This allows your camera to be operated in fast stream modes.

Device drivers are included in most commercial imaging packages to assist registration and to allow realtime and off-line ratioing or fluorescence overlays. Alternatively the TripleSplit can be used with simple image capture software and the processing carried out manually offline or using our own MicroManager and ImageJ drivers. The simple and accessible design makes the Optosplit III an excellent platform for alternative applications, such as pFRET polarisation, or multi-depth imaging.

Whilst optimised for coupling to a scientific microscope, the Optosplit III image splitters can also be used with camera lenses or any other system of lenses which produce an image plane of suitable size and f/number (please ask for details).

 

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Additional information

Applications

• Förster Resonance Energy Transfer (FRET)
• Ratiometric calcium, volltage & pH imaging
• Simultaneous multi fluorescent probe imaging
• Total Internal Reflection Fluorescence (TIRF)
• Polarisation studies (anisotropy)
• Spinning disk confocal
• Super resolution STORM / PALM / SIM
• Single Plane Illumination Microscopy (SPIM)
• Simultaneous multi depth imaging (using independent lenses)
• 3D super resolution PALM/STORM (using cylindrical lenses)
• Polarisation Förster Resonance Energy Transfer (pFRET)
• Simultaneous phase contrast / DIC and fluorescence

Features

• Compact design with integral C-mount input and output ports (optional T or F mount)
• Simple & precise controls for image registration
• Interchangeable filter / dichroic holders
• Bypass without removing from optical path
• Rotating filter mount for polarisation studies
• 1X or 1.3X magnification
• Support for sensors up to 18.8mm diagonal (13.3mm x 13.3mm sensors)
• Angled and flat auxiliary drop ins for neutral density filtering, polarisers or chromatic correction
• Supports 25mm filters and standard dichroic sizes
• 40mm diameter optics
• 425nm to 875nm coatings on all surfaces
• Fixed or variable centre fully adjustable rectangular mask to delimit region of interest

Recent Publications

1) Membrane lipid order of sub-synaptic T cell vesicles correlates with their dynamics and function – Read Here
2) Recruitment of UvrBC complexes to UV-induced damage in the absence of UvrA increases cell survival – Read Here
3) Connecting Protein Conformation and Dynamics with Ligand-Receptor Binding Using Three-Color FRET Tracking – Read Here
4) Multiphoton light-sheet microscopy using wavelength mixing: fast multicolor imaging of the beating Zebrafish heart with low photobleaching – Read Here
5) The Mechanical Properties of Early Drosophila Embryos Measured by High-Speed Video Microrheology – Read Here
6) Mto2 multisite phosphorylation inactivates non-spindle microtubule nucleation complexes during mitosis – Read Here