OptoSplit III

• Three way emission image splitter
• 1, 2 or 3 images on a single camera
• Large sensor sCMOS format

Product Description

The OptoSplit III (TripleSplit) extends the OptoSplit image splitter concept, adding an optional second beamsplitter to split the field into either two or three separate, spatially equivalent, channels, which can be displayed side by side on a single camera chip.

Splitting is usually performed on the basis of wavelength or polarisation, allowing applications where there is a requirement for simultaneous, or high speed, acquisition of multiple emission bands or polarisations states. The simultaneous acquisition of up to three images offers a major benefit over manual or electronic filter changers, as there is no longer a need to pause acquisition while the filter position is changed. This allows your camera to be operated in fast stream modes.

Device drivers are included in most commercial imaging packages to assist registration and to allow realtime and off-line ratioing or fluorescence overlays. Alternatively the TripleSplit can be used with simple image capture software and the processing carried out manually offline or using our own MicroManager and ImageJ drivers. The simple and accessible design makes the Optosplit III an excellent platform for alternative applications, such as pFRET polarisation, or multi-depth imaging.

Whilst optimised for coupling to a scientific microscope, the Optosplit III image splitters can also be used with camera lenses or any other system of lenses which produce an image plane of suitable size and f/number (please ask for details).

 

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Additional information

Applications

• Förster Resonance Energy Transfer (FRET)
• Ratiometric calcium, voltage & pH imaging
• Simultaneous multi fluorescent probe imaging
• Polarisation studies (anisotropy)
• Simultaneous phase contrast / DIC and fluorescence
• Simultaneous multi Z depth imaging
• Total Internal Reflection Fluorescence (TIRF)
• Spinning disk confocal
• Single Plane Illumination Microscopy (SPIM)
• 3D super resolution PALM/STORM (using cylindrical lenses)

Features

• Compact design with integral C-mount input and output ports as standard (F and T mount on request)
• Support for sensors up to 32mm diagonal (split mode only)
• Budget friendly alternative to three cameras
• 425nm to 875nm AR coatings on all optical surfaces
• 40mm diameter proprietary optics
• Simple & precise controls for image registration
• Interchangeable filter / dichroic holders
• Emission filter dimensions – 25mm diameter
• Recommended dichroic dimensions – 26x38x2mm (lambda/2 flatness)
• Single channel ‘Bypass’ cube to easily switch to single channel operation
• Angled and flat auxiliary drop-in holders for 25mm ND filters and chromatic correction lenses (1 inch on request)
• 1x and 1.3x magnification available
• Optional polarisation splitting kit including beamsplitter cube and rotatable polariser
• Fixed or variable centre fully adjustable rectangular mask to delimit region of interest

Additional information

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Recent publications

2021

2020

  • Forces during cellular uptake of viruses and nanoparticles at the ventral side – Read here
  • Direct observation of dynamic protein interactions involving human microtubules using solid-state NMR spectroscopy – Read here

2019

  • Defects in assembly explain reduced antiviral activity of the G249D polymorphism in human TRIM5α – Read here 
  • Simultaneous Dual Optical Mapping of the Heart Using a Single Excitation, Single Camera Approach – Read here 
  • Age-dependent changes in electrophysiology and calcium handling – implications for pediatric cardiac research – Read here 
  • E-syt1 Re-arranges STIM1 Clusters to Stabilize Ring-shaped ER-PM Contact Sites and Accelerate Ca2+ Store Replenishment – Read here