OptoSplit II

• Dual emission image splitter
• 1 or 2 images on a single camera
• Large sensor (sCMOS) format

Product Description

Dual Emission Image Splitter

The Cairn OptoSplit II emission image splitter is a simple device enabling a single camera to record images simultaneously at two different optical wavelengths, polarisation states or other differentiated states.

Traditionally, dual channel imaging is performed using an electronic filter changer or an additional camera and beamsplitter, neither of which is ideal for all applications. The switching speed of an electronic filter changer limits the temporal resolution, whereas a second camera adds cost and complexity. The OptoSplit uses a unique rotating mirror cradle, which gives adjustable spatial separation, to ensure excellent image registration and features a fully adjustable rectangular aperture to enable cropped sensor imaging modes and reduced scatter.

The latest version uses our own lens design to support the Larger Sensors of scientific CMOS and some EMCCD cameras. The instruments have a correspondingly larger aperture and improved off-axis correction to give enhanced performance with all sensors.

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Device drivers are included in most commercial imaging packages to assist registration and to allow real-time and offline ratioing, or fluorescence overlays. Alternatively, the OptoSplit can be used with simple image capture software and the processing carried out manually offline, or using our own MicroManager and ImageJ drivers. The simple and accessible design makes the OptoSplit II  an excellent platform for alternative applications, such as dual polarisation imaging.

Whilst optimised for coupling to any scientific microscope, the image splitters can also be used with camera lenses or any other system of lenses which produce an image plane of suitable size and f/number (please ask for details).

Download Data Sheet Manuals video support Get a Quote Comparisons Back to Image-Splitters

Additional information


• Förster Resonance Energy Transfer (FRET)
• Ratiometric calcium, voltage & pH imaging
• Simultaneous multi fluorescent probe imaging
• Total Internal Reflection Fluorescence (TIRF)
• Spinning disk confocal
• Single Plane Illumination Microscopy (SPIM)
• Polarisation studies (anisotropy)
• 3D super resolution PALM/STORM (using cylindrical lenses)
• Simultaneous phase contrast / DIC and fluorescence


• Compact design with integral C-mount input and output ports
• Simple & precise controls for image registration
• Interchangeable filter / dichroic holders
• Rotating filter mount for polarisation studies
• 1X, 1.3X and 1.7X magnification available
• Support for sensors up to 18.8mm diagonal (13.3mm x 13.3mm sensors)
• Angled and flat auxiliary drop ins for neutral density filtering, polarisers or chromatic correction
• Supports 25mm filters
• Supports 2mm thick Ultraflat dichroics along with our recommended size (26x38x2mm)
• 40mm diameter proprietary optics
• 425nm to 875nm coatings on all surfaces

Recent Publications


  • Age-dependent changes in electrophysiology and calcium handling – implications for pediatric cardiac research – Read here
  • A Protocol for Transverse Cardiac Slicing and Optical Mapping in Murine Heart – Read here
  • A nanofluidic device for real-time visualization of DNA–protein interactions on the single DNA molecule level – Read here
  • Imipramine as an alternative to formamide to detubulate rat ventricular cardiomyocytes – Read here
  • Mechanism of 17β-estradiol stimulated integration of human mesenchymal stem cells in heart tissue – Read here
  • Structural and Functional Determinants of AC8 Trafficking, Targeting and Responsiveness in Lipid Raft Microdomains – Read here

Pre 2019

  • The architecture of EGFR’s basal complexes reveals autoinhibition mechanisms in dimers and oligomers – Read Here
  • Combining Gold Nanoparticle Antennas with Single Molecule Fluorescence Resonance Energy Transfer (smFRET) to Study DNA Hairpin Dynamics – Read Here

Investigation of the flow structure in thin polymer films using 3D µPTV enhanced by GPU – Read Here4) Single-Molecule FRET Assay to Observe the Activity of Proteins Involved in RNA/RNA Annealing – Read Here5) Single-Particle Tracking of Cell Surface Proteins – Read Here
6) Super-Resolution Monitoring of Mitochondrial Dynamics upon Time-Gated Photo-Triggered Release of Nitric Oxide – Read Here
7) Kinetic analysis of single molecule FRET transitions without trajectories – Read Here
8) Studying Structural Dynamics of Potassium Channels by Single-Molecule FRET – Read Here
9) Single-Molecule Fluorescence Microscopy in Living Caenorhabditis elegans – Read Here
10) Role of FK506-binding protein in Ca2+ Spark regulation – Read Here