OptoSplit II Ex-Demonstration

• Dual emission image splitter
• 1 or 2 images on a single camera
• Large sensor (sCMOS) format

Product Description

Dual Emission Image Splitter

The Cairn OptoSplit II emission image splitter is a simple device enabling a single camera to record images simultaneously at two different optical wavelengths, polarisation states or other differentiated state.

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Traditionally, dual channel imaging is performed using an electronic filter changer or an additional camera and beamsplitter, neither of which is ideal for all applications. The switching speed of an electronic filter changer limits the temporal resolution, whereas a second camera adds cost and complexity. The OptoSplit uses a unique rotating mirror cradle, which gives adjustable spatial separation, to ensure excellent image registration and features a fully adjustable rectangular aperture to enable cropped sensor imaging modes and reduced scatter.

The latest version uses our own lens design to support the Larger Sensors of scientific CMOS and some EMCCDs cameras. The instruments have a correspondingly larger aperture and improved off-axis correction to give enhanced performance with all sensors.

Device drivers are included in most commercial imaging packages to assist registration and to allow real-time and offline ratioing, or fluorescence overlays. Alternatively the OptoSplit can be used with simple image capture software and the processing carried out manually offline or using our own MicroManager and ImageJ drivers. The simple and accessible design makes the OptoSplit II LS an excellent platform for alternative applications, such as dual polarisation imaging.

Whilst optimised for coupling to any scientific microscope, the OptoSplit can also be used with camera lenses or any other system of lenses which produce an image plane of suitable size and f/number (please ask for details).

Recent Publications

1) Studying Structural Dynamics of Potassium Channels by Single-Molecule FRET – Read Here

2) Single-Molecule Fluorescence Microscopy in Living Caenorhabditis elegans – Read Here

3) Role of FK506-binding protein in Ca2+ Spark regulation – Read Here

4) Spectroscopic imaging studies of nanoscale polarity and mass transport phenomena in self-assembled organic nanotubes – Read Here

5) Multi-colour direct STORM with red emitting carbocyanines – Read Here


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Additional information


• Förster Resonance Energy Transfer (FRET)
• Ratiometric calcium, voltage & pH imaging
• Simultaneous multi fluorescent probe imaging
• Total Internal Reflection Fluorescence (TIRF)
• Spinning disk confocal
• Single Plane Illumination Microscopy (SPIM)
• Polarisation studies (anisotropy)
• 3D super resolution PALM/STORM (using cylindrical lenses)
• Simultaneous phase contrast / DIC and fluorescence


• Compact design with integral C-mount input and output ports
• Simple & precise controls for image registration
• Interchangeable filter / dichroic holders
• Bypass without removing from optical path
• Rotating filter mount for polarisation studies
• 1X or 1.3X magnification
• Support for Large Sensors – 16.6 x 14 mm
• Angled and flat auxiliary drop ins for neutral density filtering, polarisers or chromatic correction
• Supports 25mm filters
• Supports 2mm thick Ultraflat dichroics along with our recommended size (26x38x2mm)
• 40mm diameter proprietary optics
• 450nm to 875nm coatings on all surfaces