OptoSplit II ByPass Image Splitter

• Two way emission image splitter with bypass
• 1 or 2 images on a single camera
• Large sensor (sCMOS) format

Product Description

Enhanced long-term stability, pixel registration and reproducibility

The Cairn OptoSplit II BP is our best ever dual channel simultaneous imaging device for use with a single camera. It builds on the success of the OptoSplit II image splitter, but adds a convenient single lever bypass mode making it more suitable for multi-user microscopes where simultaneous dual channels are only required for specific experiments alongside single wavelength recordings.

Whilst maintaining compatibility with the OptoSplit II, the BP version now supports our new flat-face filter cubes and has enhanced long-term stability, pixel registration and reproducibility. Featurewise, the rapid Bypass control is complimented by additional space for more auxiliary components. It has a slightly larger footprint than the OptoSplit II and consequently can use longer focal ratio lenses with even better off-axis performance.

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Device drivers are included in most commercial imaging packages to assist registration and to allow real-time and off-line ratioing or fluorescence overlays. Alternatively the OptoSplit II BP can be used with simple image capture software and the processing carried out manually offline or using our own MicroManager and ImageJ drivers. The simple and accessible design makes the OptoSplit II BP an excellent platform for alternative applications, such as dual polarisation imaging.

Whilst optimised for coupling to a scientific microscope, the OptoSplit II BP image splitters can also be used with camera lenses or any other system of lenses which produce an image plane of suitable size and f/number (please ask for details).

Download Data Sheet OptoSplit II Bypass Manual video support Get a Quote Comparisons Back to Image-Splitters

Additional information


• Förster Resonance Energy Transfer (FRET)
• Ratiometric calcium, volltage & pH imaging
• Simultaneous multi fluorescent probe imaging
• Total Internal Reflection Fluorescence (TIRF)
• Spinning disk confocal
• Single Plane Illumination Microscopy (SPIM)
• Super resolution STORM / PALM / SIM
• Polarisation studies (anisotropy)
• Simultaneous dual depth imaging (using independent lenses)
• 3D super resolution PALM/STORM (using cylindrical lenses)
• Simultaneous phase contrast / DIC and fluorescence

Key Benefits

• Compact design with integral C-mount input and output ports (optional T or F mount)
• Simple & precise controls for image registration
• Interchangeable filter / dichroic holders
• Single lever switching from split to bypass
• Rotating filter mount for polarisation studies
• 1X or 1.3X magnification
• Support for sensors up to 18.8mm diagonal (13.3mm x 13.3mm sensors)
• Angled and flat auxiliary drop ins for neutral density filtering, polarisers or chromatic correction
• Supports 25mm filters and standard microscope dichroic sizes
• 40mm diameter optics
• 425nm to 875nm coatings on all surfaces
• Fixed or variable centre fully adjustable rectangular mask to delimit region of interest

Additional information

Recent articles

Multifocus imager theory and practice

Multifocus imager calculations spreadsheet

Super resolution application note

Recent publications


Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568


  • E-syt1 Re-arranges STIM1 Clusters to Stabilize Ring-shaped ER-PM Contact Sites and Accelerate Ca2+ Store Replenishment – Read here 
  • Spatiotemporal Regulation of Myosin II Dynamics during Cell Movement – Read here 
  • 3D super-resolution microscopy performance and quantitative analysis assessment using DNA-PAINT and DNA origami test samples – Read here