Light tight enclosure for sensitive optical recordings.
The Cairn Alligator* system allows bioluminescence measurements to be simultaneously recorded from up to six multi-well plates over several days. Based on the New Brunswick Galaxy 170R incubator, we make substantial modifications to convert the standard incubator into a high throughput luminometer. In addition to the precise temperature, O2, CO2 and humidity control provided as standard, we adapt the chamber for light-tightness and high NA imaging using a deep-cooled, back-thinned or Electron Multiplied CCD. The system is supplied fully integrated into various software packages for multi-region timelapse analysis.This fantastic new system offers the capacity for high-throughput collection over time scales that vary from seconds to weeks, without sacrificing the quality of the data collected. The flexible and sensitive environmental control gives us the ability to do experiments which would simply not be possible otherwise.
A jove video showing how the ALLIGATOR can be used in the context of circadian rhythms research (thanks to John O’Neill’s lab, Cambridge)