Light tight enclosure for sensitive optical recordings.
The ALLIGATOR comprises of a high-sensitivity EMCCD camera mounted with high resolution and anti-mist optics enclosed within a custom humidified CO2/N2 170 L tissue culture incubator and light-tight shroud. The ALLIGATOR can image up to six 96-well plates simultaneously and supports recording from a wide variety of cell types, tissues, and organotypic slices. In addition, the ALLIGATOR affords programmable control of temperature, % CO2, and % O2 allowing long-term bioluminescence recordings under physiologically relevant conditions. The system is fully supported in all commercial software imaging packages (Micromanager, Visiview, NIS-Elements, etc).This fantastic new system offers the capacity for high-throughput collection over time scales that vary from seconds to weeks, without sacrificing the quality of the data collected. The flexible and sensitive environmental control gives us the ability to do experiments which would simply not be possible otherwise.
A jove video showing how the ALLIGATOR can be used in the context of circadian rhythms research (thanks to John O’Neill’s lab, Cambridge)