The various parts that need to be fitted to a microscope frame for phase contrast microscopy tend to be rather bulky. This classical arrangement for phase contrast imaging often restricts access to the sample from above on an inverted microscope frame, preventing the end user from applying certain experimental techniques. One such technique is Scanning Ion Conductance Microscopy (SICM) where an electrode is positioned immediately above the sample and can be used to map its surface profile without needing to be in direct physical contact with it.
Dr Gabriel Meloni (Warwick University) is interested in multimodal microscopy and wanted to find a way to combine SICM and phase contrast microscopy to monitor bacterial spore germination. It would not be possible to do this using a conventional phase contrast assembly on an inverted microscope frame because the component parts would hinder the precise positioning of the SICM electrode tip. Furthermore, the electrode assembly itself would block light from the transmitted light source reaching the sample, thereby degrading image quality.