The DAPI-FITC-TRITC triple band fluorescence filter combination is designed for simultaneous detection of the fluorochromes DAPI, FITC, and TRITC with minimal crossover (spectral bleed-through) between adjacent bands, and it can also be employed with other combinations of fluorescent probes that have similar spectral profiles. This filter set selects for the following wavelengths: violet excitation and blue emission detection, blue excitation and green emission detection, and green excitation coupled to orange-red emission.