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CFP/YFP
FRET measurement between YFP and CFP
EYFP and ECFP function as a donor-acceptor pair for fluorescence resonance energy transfer (FRET), in which excitation of the donor (cyan) molecule leads to emission from the acceptor (yellow) molecule, provided that the proteins are close enough for energy transfer to occur. FRET can therefore be used to monitor direct protein-protein interactions between EYFP and ECFP fusion proteins in living or fixed cells
(for review see Pollock and Heim, 1999).
Pollok, B.A., and R. Heim. 1999. Using GFP in FRET-based applications. Trends Cell Biol. 9:57-60.
To the right you can see the filter configuration for CFP-YFP (click to enlarge) with further information in the data sheet, whilst the LED curves lie below.
The system includes:
Cyan (505)
White
