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OptoLED light source
LED light source
The OptoLED is our flagship system for LED illumination.
The system has key advantages in live-cell recordings where long term drift in intensity can be a problem with alternative light sources. The ultra-high stability and “instantaneous” vibration-free switching and intensity modulation are valuable in a wide range of applications such as combined imaging and electrophysiology, voltage sensitive dye studies and high-speed tracking. The facility to “gate” the light source directly from the camera and thus only expose the specimen whilst the camera is integrating helps to reduce photobleaching and also prevents bleedthrough during frame readout.
Ultraviolet and white LEDs are also available for uncaging applications and brightfield illumination.
Applications
- Fluorescence Microscopy
- Live-cell Imaging
- Uncaging
- Macroscopic Imaging
Key benefits
- Dual power supply with photodiode feedback loop for absolute short and long term stability
- Sub microsecond digital switching and external intensity control
- Current and power metering with overload protection
- Modular LED heads covering the ultraviolet and visible spectrum
- Multiport couplings to all popular fluorescence microscopes
- Overdrive facility for extra intensity during discontinuous operation
Wavelength Options
The range of wavelengths available from ultra-bright LEDs covers the absorbance peaks of most fluorescence indicators. The table below shows the standard range, however we can also source more specialist devices.
Depending on the combination of wavelengths required we can combine two or more devices using dichroic mirrors. Please contact Cairn for recommendations on the best LEDs and filters for your experiments.
| Wavelength |
Product Description |
|---|---|
| 365nm | ultraviolet |
| 385nm | ultraviolet |
| 405nm | violet |
| 440nm | white short |
| 455nm | royal blue |
| 470nm | blue |
| 505nm | cyan |
| 530nm | green |
| 550nm | white long |
| 580nm | warm white |
| 590nm | amber |
| 550nm | white long |
| 627nm | red |
| 660nm | red (long) |
| 850nm | infra red |
| 950nm |
infra red |
For data on our selection of LEDs please click here
If only single wavelength illumination is needed try the OptoLED Lite
LED head upgrade
With LED technology constantly improving we would like to remind existing customers that we are able to upgrade your current LED heads for a small charge of £95 each.
The way in which our LED heads are designed means they are readily interchangable so if returned to us we can upgrade in a very short period of time.
We regret that this product cannot be sold in the USA for microscope epi-fluorescence illumination applications
