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Ultra Stable and Instantaneous Wavelength Switching
Because of possible patenting issues, the OptoLED and OptoLED Lite products are currently not available for fluorescence microscopy applications in North America.

Recent advances in Light Emitting Diode (LED) technology has produced devices that now offer a viable alternative to arc and halogen light sources in microscopy and related techniques. Our OptoLED power supply and modular LED head system provides an off-the-shelf solution for fluorescence illumination, delivering dramatically improved lifespan, switching speeds and stability.

The system has key advantages in live-cell recordings where long term drift in intensity can be a problem with alternative light sources. The ultra-high stability and “instantaneous” vibration-free switching and intensity modulation are other key benefits in a wide range of applications such as combined imaging and electrophysiology, voltage sensitive dye studies and high-speed tracking. The facility to “gate” the light source directly from the camera and thus only expose the specimen whilst the camera is integrating helps to reduce photobleaching and also prevents bleedthrough during frame readout.

Ultraviolet and white LEDs are also available for uncaging applications and brightfield illumination.

Applications
  • Fluorescence Microscopy
  • Live-cell Imaging
  • Uncaging
  • Macroscopic Imaging
  • Two channel power supply incorporating photodiode feedback loop for absolute short and long term stability
  • Flexible interface with logic level TTL switching and front panel or external intensity control
  • Current and power metering with overload protection
  • Wide choice of modular LED heads covering the ultraviolet and visible spectrum
  • Single and dual couplings to all popular fluorescence microscopes
  • Sub-microsecond switching
  • Overdrive facility for discontinuous operation
More Information...
Wavelength Options
The range of wavelengths available from ultra-bright LEDs covers the absorbance peaks of most fluorescence indicators. The table below shows the standard range, however we can also source more specialist devices (download information sheet).
Depending on the combination of wavelengths required we can combine two or more devices using dichroic mirrors. Please contact Cairn for recommendations on the best LEDs and filters for your experiments.
Product Code Product Description
370nm ultraviolet
400nm ultra
455nm royal blue
470nm blue
505nm cyan
530nm green
590nm amber
617nm red/orange
627nm red
OptoLED Configurations
Most of our standard configurations are based on coupling one or two LEDs to the epi-fluorescence port of a research microscope. The system is extremely flexible so please let us know if you have other requirements.
Standard Systems
Product Code Product Description
P100/CLA/000 High stability Classic arc lamp house with preset (65W - 80W) power supply (horizontal lamp mount)
P101/OPT/001 High stability Optosource arc lamphouse with variable power supply for 75W Xenon lamps
P101/OPT/002 High stability Optosource arc lamphouse with variable power supply for 150W Xenon lamps
P101/OPT/003 High stability Optosource arc lamphouse with variable power supply for 100W Mercury lamps
P101/OPT/004 High stability Optosource arc lamphouse with variable power supply for 150W Mercury / Xenon lamps
P101/OPT/005 High stability Optosource arc lamphouse with variable power supply for 100W Mercury / Xenon lamps
P101/OPT/552 High stability Optosource arc lamphouse with collimating quartz optics and variable power supply for 150W Xenon lamps.